Protein_Domain

Part:BBa_K203000

Designed by: Douaa Mugahid and Michael Bartoschek   Group: iGEM09_Heidelberg   (2009-10-15)

Plasma membrane targeting (GPI anchor)

This part encodes the attachement of a GPI anchor, which allows targetting of proteins to which it is tagged to the Plasma Membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 146
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

n/aPlasma membrane targeting (GPI anchor)

Shown to localize proteins to the plasma membrane. An expression device consisting of Part:BBa_I712004 (CMV) Part:BBa_K203103 (GFP), Part:BBa_K203000 and Part:BBa_K203102 (terminator) was assembled for this characterization.


GPI.jpg


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Categories
Parameters
n/aPlasma membrane targeting (GPI anchor)